Purpose: To compare the transport of six b-adrenergic receptor antagonists tested individually or combined (“N-in-one”) across Caco-2 cell monolayers prepared by either the Biocoat® 3 to 5-day culture method or the standard 21-day culture method.
Methods: Caco-2 cell monolayers were cultured using either the Biocoat® 3 to 5-day, or the standard 21-day methods. Monolayers prepared with the ...
In a typical cell culture process for making therapeutics, cultures need to be closely monitored for their viability, genetic stability, production potential, and for potential contamination. It is essential that the levels of key nutrients and metabolites as well as cell concentration be known. This allows the operator to maintain the culture appropriately, by using the correct feeding strategy ...
Telomerase is a specialized ribonucleoprotein composed of telomerase reverse transcriptase (TERT), an intrinsic RNA template (TR), and several associated proteins. Its main function is to stabilize telomeres, which protect chromosomes from recombination and end-to-end fusion.
Telomeres also recognize DNA damage and control the replicative capability of cells. With each cell division some of ...
The RealBio™D4 Culture System Technology Overview
INTRODUCTION The RealBio™D4 Culture System is a revolutionary new tool that permits scientists to culture tissues and cells in an in vitro arrangement that mimics the natural in vivo composition, configuration and function of tissues. The system is a valuable tool for investigators across a broad range of disciplines including cell biologists, ...
Accurate measurement of immune activity in relation to therapeutic dosing is an important part of many clinical trials, yet is poorly addressed by traditional techniques. Typical immune activity monitoring is performed using isolated leukocytes or even sorted sub- population culturing methods followed by an analytical measurement. These methods require the shipment of intact blood samples to a ...
AMSBIO have announced a range of ready-to- use lentivirus supernatant products suitable for many kinds of gene delivery applications including mammalian protein expression, stable cell line construction, cell signal pathway localization and stem cell research.
Converting fully differentiated mouse or human somatic cells into embryonic-like cells (so called induced Pluripotent Stem Cell: iPSC) ...
Process variability is a leading cause in biopharmaceutical batch failure and impacts the value and profitability of the industry. Thermo Fisher Scientific controls inherent variability through the use of the Thermo Scientific Antaris line of Fourier transform near-infrared (FT-NIR) spectrometers.
Profiling of impurities in biopharmaceuticals and their associated intermediates and excipients is a regulatory expectation. Since residuals are typically present at low levels in difficult sample matrices, development and validation of assays can be quite challenging. Matrix types can vary greatly due to the fact that sampling at a variety of process steps is required to accurately monitor ...
Vessel Preparation Cleanliness of the bottles is critical. Common tissue culture glassware cleaning procedures may be inadequate. Included in these instructions are suggested roller bottle cleaning protocols.
Sterilization Autoclave the bottle and cap as a unit with cap loosened to allow venting. Exhaust autoclave slowly, allowing bottles to cool before removing from the autoclave. Rubber liners ...
Caco-2 cells are a widely used in vitro model for studies involving the transepithelial transport of potential drug candidates. The purpose of this study was to compare the transport characteristics of six compounds, all beta- adrenergic receptor antagonists, both individually and combined (“N-in-one”) across Caco-2 cell monolayers. Caco- 2 cells were cultured on Costar Transwell inserts in ...
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