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Polyclonal Antibodies

    
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IMGENEX  Antibodies, protein expression systems, and tissue microarrays for use in studies of cancer, apoptosis, molecular signaling pathways, innate immunology
 
Advantages of IMGENEX GeneSuppressor™ Kits • No worries about RNA degradation • Cost effective analysis • Allows sustained silencing of protein production • Generation of permanent cell lines may...
 
Available Antibodies from IMGENEX In recent years, the study of cancer biology has elicited a close look at key factors involved in the regulation of cell cycle. In particular, much...
 
United States Biological  USBiological is a long-established biotech manufacturer located near Boston, offering a wide range of biologicals, biochemicals and the largest selection of antibodies you will find anywhere. USBio offers over 100,000...
 
A0888-35F Triglycerides are the most efficient form of energy storage in mammalian adipose tissue during times of caloric excess. ATGL is one of the key enzymes...
 
A1059-62 Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia, and some cancers are associated with obesity. An...
 
AbD Serotec  Leading global antibody supplier. AbD Serotec’s product range includes apoptosis kits, autophagy reagents, CD marker antibodies, epitope tag antibodies, immunology antibodies, neuroscience antibodies, stem cell...
 
AQUAPORIN 2 (Polyclonal Antibody) Target: Rat Host: Rabbit Reactivity: Mouse, Dog, Primate, Human, Bovine, Chicken
 
ARG VASOPRESSIN RECEPTOR (Polyclonal Antibody) Target: Rat Host: Rabbit Reactivity: Bovine, Human, Pig, Mouse
 
LI-COR Biosciences  LI-COR Biosciences’ near-infrared fluorescent and chemiluminescent imaging systems, reagents and accessories are ideal for today’s cancer research. Includes Odyssey, Odyssey Fc, Pearl, 4300 DNA Analyzer, BrightSite...
 
beta-Tubulin Rabbit Antibody, 100 µl This beta-tubulin primary antibody can be used for detection of beta-tubulin by Western blot or by In-Cell Western™ Assay. Detection of this primary...
ProSci Incorporated  Antibody Supplier: Affinity-purified antibody, peptide-specific polyclonal antibodies to novel antigens, monoclonal antibody production, polyclonal antibody production, lysates, immunoblots, recombinant proteins
 
1110033M05RIK Antibody The amino acid sequence of 1110033M05Rik is derived from an annotated genomic sequence (NT_039589) using gene prediction method: GNOMON, supported by mRNA...
 
1110051B16RIK Antibody cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and...
 
Acris Antibodies Inc.  Acris GmbH was founded 1998 as a spinoff of DPC Biermann GmbH, the German subsidiary of Diagnostic Products Corp., Los Angeles. Acris took over responsibility for marketing the DPC Biermann research products. From 1999 to...
 
Antibody Offerings In the final stage we achieved in 2010 we were able to offer more than 170,000 primary antibodies partly sold as Acris own products partly offered as...
 
R1091AP GFP antibody Goat anti A. victoria GFP. Presentation: AP Product is tested for Frozen sections, Enzyme Immunoassay, Western blot / Immunoblot.
Randox Life Sciences  Randox Life Sciences is a recognised primary manufacturer of high quality and cost-effective human recombinant proteins, polyclonal and monoclonal antibodies providing custom antibody production.
 
17a-ESTRADIOL Boldenone is an anabolic steroid which is administered as boldenone undecylenate and is approved by the United States FDA for use in horses not intended for...
 
17a-NORTESTOSTERONE 17β, 19-nortestosterone, commonly known as nandrolone, is an anabolic androgen given by mouth and by injection. It is a natural hormone in some animal...
 
Biomatik Corporation  Biomatik is the world's leading supplier of custom services including custom gene, peptide, antibody and protein synthesis as well as life science products including Biochemicals, Enzymes and PCR products
 
Anti-V5 Tag polyclonal Antibody, Goat Anti-V5 Tag polyclonal Antibody, Goat
 
Custom Polyclonal Antibody Production Custom Polyclonal Antibody Production that are dependable and affordable!
Capralogics Inc.  Capralogics is a full service polyclonal antibody production facility. For over 15 years we have been dedicated to providing high quality animal serum and plasma for diagnostic, research, and discovery applications....
 
5-Methyl Cytosine 5-Methyl Cytosine
 
5-Methyl Cytosine Sheep anti-5-methyl cytosine 250ug
 
Maine Biotechnology Services  Maine Biotechnology Services provides custom monoclonal antibody development, polyclonal antibody development, matched pair analysis, bulk antibody production, antibody purification services, and assay development...
 
Antibody Production Services- Maine Biotechnology Services Maine Biotechnology Services offers both in vivo and in vitro antibody production in our cGMP facility at pilot and bulk scales. In Vivo Monoclonal...
 
Antibody Purification Services- Maine Biotechnology Services Murine Monoclonal IgGs are purified by Protein A affinity chromatography, eluting by pH gradient to ensure gentlest conditions possible. Service includes...
 
COVALAB  Custom antibody services : Development of Monoclonal and Polyclonal Antibodies-Peptide synthesis...Products :primary antibodies -Secondary Antibodies-Diagnostic kits - Functionalizated microtitration plates...
 
Anti-Chicken IgY, Peroxydase conjugate antibody Anti-Chicken IgY, Peroxydase conjugate antibody
 
Anti-Goat IgG (H+L), Alkaline phosphatase conjugate antibody Rabbit anti-goat IgG (H+L) F(ab')2 fragment
 
SDIX  antibody development, antibody production, monoclonal antibody, polyclonal antibodies, critical reagents, assay development, immunoassay development, antigen design, genomic antibody technology, GAT, genomic antibodies,...
 
Large Scale Production To provide maximum antibody yield during production, SDIX examines the viability of your cell line and the clonality of your cell line. Clonality is the...
 
Polyclonal Antibody Production We offer polyclonal antibodies from many different species which include Rabbit, Goat, Sheep, Chicken, Mouse, Guinea Pig, and Hamster. Polyclonal antibodies...
Denovo Biotechnology  Custom monoclonal antibodies, custom polyclonal antibodies from mouse, goat, sheep, chicken, donkey and llama. Protein expression, production scale-up, purification and characterization services, Molecular biology...
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Polyclonal Antibodies - Product Reviews

Exciting new packaging materials for polyclonal antibodies: chicken eggs

A wide range of animals are used in the production of polyclonal antibodies. Although mammals are most commonly used, "These data suggest that chickens are an alternative model for producing pAbs (polyclonal antibodies) against mammal high-homology proteins such as MT (metallothioneins)" reports Angélica María Ortiz-Bueno, et al in Applied Biochemistry and Biotechnology, online issue 1559-0291.

Chicken hosts have several advantages, the most interesting of which is the fact that the antibodies are conveniently packaged in the chicken eggs, which can be stored for a long time. Other advantages include the fact that chickens produce 10 to 20 times more chicken egg yolk immunoglobulins (IgY) and are less expensive to maintain than rabbits and, while IgG made from a recombinant protein using a mammalian host takes 6 to 9 month to produce, IgYs only take 3 months. In addition, 300 million years separate chickens from the human evolutionary line in contrast to rabbits and mice with only 60 million years of separation. The result is that chickens generally mount a more vigorous immune response to most human gene products, including highly conserved ones.

Last but not least, the controversial subject of animal rights can be avoided by using chickens rather than mammals because, instead of having to be bled to yield the product, chickens deliver the polyclonal antibodies by simply doing what they do best: laying convenient, long-lasting sealed containers, in other words, eggs.


Best-in-Class Polyclonal Antibody Producers Who Use Chickens Feature:

  • Use of in-bred strains which have higher antibodies responses.
  • Immunization of birds before they begin to lay because stress of vaccination may adversely affect egg production.
  • Birds kept in housing conditions that encourage natural behavior and are protected from contamination.

Top Considerations Before Buying Polyclonal Antibodies Produced by Chickens:

Chicken IgY cannot be used in every situation so research should be done concerning whether IgY is appropriate for your particular line.


Key Producers:

1. Maine Biotechnology Services (MBS) offers complete antibody services, including hybridoma development, polyclonal antibody development, antibody production, purification and post processing. Its MultiPure technology enables customers to assess relative affinity, physical characteristics and matched pair capability before proceeding to subcloning. What sets it apart is its Academic Discovery Track, a scaled-down antibody development which is an affordable alternative for researchers and academics. MBS is continually expanding its product line and services. Recent additions include two antibodies that recognize large, small and branched versions of polyethylene glycol (PEG). This Portland, Maine-based company, founded in 1990, ships products to companies around the United States and in the European Union.

2. AMS Biotechnology (Europe) Ltd (amsbio) provides life science research reagents and services to small and medium-sized manufacturers. Its expanding catalogue contains 285,000 products. amsbio, which was founded in 1987 and restructured in 1997, specializes in Genomics, Proteomics and Immunology. This ISO 9001:2008 registered company offers custom polyclonal and monoclonal development services as well as thousands of protein specific polyclonal primary antibodies and isotype specific secondary antibodies conjugated with enzymatic and fluorescent indicators. Future plans include adding DNA damage screening, Cell Assays, Tumour Xenograft, and primate and rodent services. ambios is able to ship products to companies around the world from its offices in Switzerland, Germany and the UK.

3. United States Biological markets biochemicals, polyclonal antibodies, recombinant proteins, cell culture media and molecular biology kits to pharmaceutical and biotechnology companies in the United States and around the world. Customer service and technical support are available worldwide. US Biological markets 150,000+ products, but what sets it apart is its approach to customer helpdesk protocol. President Warren Shore says that, "We believe in answering all inquiries within a few hours of receipt. There is no voice mail at United States Biological...you always get to speak with humans..." Its headquarters are in Swampscott, Massachusetts.

4. Trevigen, Inc. is a privately-held biotechnology company that specializes in cancer research products, with an emphasis on apoptosis, DNA damage and repair and cancer cell function and behavior. This company was founded in 1992 and has a single office in Gaithersburg, Maryland. In 2008, it opened a new division, Trevigen Cell Assays, (TCA)  to offer contract research work to the pharmaceutical and biotechnology industries as well as government and academic research institutions, specializing in designing and conducting assays for lead compound and genotoxic screening based on DNA damage and repair and cancer cell behavior.

Polyclonal antibodies
Definition: Polyclonal antibodies (pAB) are a combination of immunoglobulin molecules that have been secreted in response to a specific antigen. The antigen is injected into an animal, inducing the B-lymphocytes to produce immunoglobulins specific for the antigen.

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Polyclonal Antibodies Research Library

Western Blotting Handbook
AbD Serotec
Since Western blotting is one of the most prevalent laboratory procedures in use today, we have produced this handy reference guide to provide a basic overview of the key technical considerations that go into performing successful Western blots. Beyond basic theory, we have included some typical experiments, as well as troubleshooting advice and time-saving pointers to make things go more ...
 
What are Polyclonal Antibodies?
Randox Life Sciences
A single immunoglobulin molecule is shown above, with the five main regions FAB, FC, Heavy chain, Light Chain and Complimentary Determining Regions (CDR). The FC region is mainly involved in compliment attachment via later stages of the immune response, which results in clearance of the antigen that the antibody has bound to. The FAB is mainly involved in scaffolding and orientating the CDR to ...
 
Poster: Protein Levels and mRNA Knockdown: The importance of accurate protein quantification in RNA silencing using PCR, Western
LI-COR Biosciences
High quality quantitative analysis of RNA silencing was obtained using two applications of the Odyssey® Infrared Imaging system; the quantitative western blot and the In-Cell Western™ (ICW) assay. Cells transfected with Akt1 siRNA or scrambled siRNA (NS1) were subjected to both quantitative real time PCR (qRT-PCR) and quantitative Western blot analysis. Following Akt1 siRNA transfection, the Akt1 ...
 
Polyclonal Antibody Production
ProMab Biotechnologies, Inc
ProMab Biotechnologies, Inc. _____________________________ Phase I Immunization and anti-sera production ! Conjugated peptides or recombinant proteins are used to immunize two rabbits. ! Injections done every 2 weeks (4-6 injections) ! Pre-immune (5ml) and immune bleeds (100-120ml antiserum) are collected ! ELISA (and Western blot if protein antigen) to determine the titer ! Approximately 3 mg ...
 
Improved Methods for Characterization of anti-Host Cell Protein Antibodies
Cygnus Technologies
Despite many acknowledged limitations, 2 Dimensional Western blot (2D WB) has historically been used to characterize the reactivity of polyclonal antibodies to individual HCPs. These antibodies are ultimately used in an orthogonal ELISA method as the final release test for total HCP owing to the much better sensitivity of ELISA. Because 2D WB lacks sufficient sensitivity and specificity to ...
 

Polyclonal Antibodies Video

Immune Response, Toll Like Receptors (TLR) Pathway - IMGENEX IMGENEX
 
Products Randox Life Sciences
 
Polyclonal Antibodies AbD Serotec Information

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Polyclonal Antibodies Acris Antibodies Inc. Products
Polyclonal Antibodies United States Biological Company
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