Samplicity™ System Delivers High-Performance Multi-sample Filtration While Streamlining Preparation of Chromatography Samples - Sponsored Whitepaper

The Samplicity™ filtration system has helped researchers streamline chromatographic sample preparation in order to match the speed and throughput requirements of their analytical separations such as high/ultrahigh performance liquid chromatography (HPLC /UHPLC). An easy-to-use alternative to syringe filters, the Samplicity™ system enables simultaneous vacuum filtration of up to eight samples. Even difficult-to-filter samples with high viscosity or particulates can be processed in seconds. Our data show that the Samplicity™ system facilitates preparation of chromatography samples with high recovery, low extractables and very low passage of particulate impurities.

Sample preparation prior to analysis helps to bring a sample to a format that is compatible with the analytical technique, reduces sample complexity, removes interfering impurities from the matrix and thereby concentrates the analyte prior to analysis. A typical sample for HPLC / UHPLC needs to be particle-free and completely soluble in the solvent compatible with the chromatography system.

Due to its simplicity and effectiveness, membrane filtration is a common sample preparation technique; however, it is one of the most neglected steps in sample prep optimization. Not enough attention is paid to filtration and the choice of filtration devices and materials, leading to inconsistency and even failure of the downstream analysis.

Sample filtration prior to chromatography is most commonly performed using syringe filtration. This is a serial, manual process, involving filtration of one sample at a time. Syringe filtration can also be very labor intensive depending on the sample type; samples which are difficult to filter (such as particle laden or viscous samples) require even higher pressures to filter, which can lead to fatigue. Syringe filtering one or two samples a day may present a mere inconvenience, but filtering large numbers of samples at a time can lead to severe fatigue, musculoskeletal pain or repetitive stress injuries.

Simplifying sample preparation, the Samplicity™ system enables filtration of up to 8 samples directly into standard HPLC vials (12 x 32 mm) using vacuum- driven filtration. This avoids multiple transfers that are